Human IL-23R/Interleukin-23 receptor ELISA Kit PicoKine®

Interleukin-23 subunit alpha is a protein that in humans is encoded by the IL23A gene. The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 355 334 354 307 337 19.5 5.7% Sample 2 1260 1269 1408 1249 1296 64.76 4.9% Sample 3 6219 5300 5785 5269 5643 390.34 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 355 334 354 307 337 19.5 5.7% Sample 1 355 334 354 307 337 19.5 5.7% Sample 2 1260 1269 1408 1249 1296 64.76 4.9% Sample 2 1260 1269 1408 1249 1296 64.76 4.9% Sample 3 6219 5300 5785 5269 5643 390.34 6.9% Sample 3 6219 5300 5785 5269 5643 390.34 6.9%

Cell Type Markers, Cytokines, Epigenetics and Nuclear Signaling, Immunoglobulins, Immunology, Innate Immunity, Interleukins, Nuclear Signaling, Nuclear Signaling Pathways, Receptors, Signal Transduction, Signaling Pathway

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 355 1260 6219 384 1339 6060 Standard deviation 19.52 79.38 478.86 28.03 99.08 545.40 CV (%) 5.5% 7.4% 7.7% 7.3% 7.4% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 355 1260 6219 384 1339 6060 Standard deviation 19.52 79.38 478.86 28.03 99.08 545.40 CV (%) 5.5% 7.4% 7.7% 7.3% 7.4% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 355 1260 6219 384 1339 6060 Mean (pg/ml) 355 1260 6219 384 1339 6060 Standard deviation 19.52 79.38 478.86 28.03 99.08 545.40 Standard deviation 19.52 79.38 478.86 28.03 99.08 545.40 CV (%) 5.5% 7.4% 7.7% 7.3% 7.4% 9% CV (%) 5.5% 7.4% 7.7% 7.3% 7.4% 9%

Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.064 0.187 0.275 0.416 0.6356 1.057 1.591 2.085 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.064 0.187 0.275 0.416 0.6356 1.057 1.591 2.085 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.064 0.187 0.275 0.416 0.6356 1.057 1.591 2.085 O.D. 0.064 0.187 0.275 0.416 0.6356 1.057 1.591 2.085

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.