sfGFP Rabbit Polyclonal Antibody
Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.
Product Specifications
CAS Number
9007-83-4
Host
Rabbit
Species Reactivity
Universal
Immunogen
Synthetic peptide immune sequence is RDHMVLHEYVNAAGITC.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB
Positive Control
GFP11-tagged fusion protein.
Concentration
1 mg/mL
Dilution
WB: 1:1,000
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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