Human Syndecan-4/SDC4 ELISA Kit PicoKine®

Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC) . The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5) -bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 82 81 78 83 6.09 7.3% Sample 2 673 735 621 630 664 45.06 6.7% Sample 3 2108 2092 1869 1903 1993 107.82 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 82 81 78 83 6.09 7.3% Sample 1 94 82 81 78 83 6.09 7.3% Sample 2 673 735 621 630 664 45.06 6.7% Sample 2 673 735 621 630 664 45.06 6.7% Sample 3 2108 2092 1869 1903 1993 107.82 5.4% Sample 3 2108 2092 1869 1903 1993 107.82 5.4%

Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Neurogenesis, Neurology Process, Neuroscience, Plasma Membrane, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 673 2108 102 680 1958 Standard deviation 3.85 52.49 128.58 5.4 56.44 154.68 CV (%) 4.1% 7.8% 6.1% 5.3% 8.3% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 673 2108 102 680 1958 Standard deviation 3.85 52.49 128.58 5.4 56.44 154.68 CV (%) 4.1% 7.8% 6.1% 5.3% 8.3% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 94 673 2108 102 680 1958 Mean (pg/ml) 94 673 2108 102 680 1958 Standard deviation 3.85 52.49 128.58 5.4 56.44 154.68 Standard deviation 3.85 52.49 128.58 5.4 56.44 154.68 CV (%) 4.1% 7.8% 6.1% 5.3% 8.3% 7.9% CV (%) 4.1% 7.8% 6.1% 5.3% 8.3% 7.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.053 0.099 0.131 0.195 0.332 0.621 1.158 2.261 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.053 0.099 0.131 0.195 0.332 0.621 1.158 2.261 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.053 0.099 0.131 0.195 0.332 0.621 1.158 2.261 O.D. 0.053 0.099 0.131 0.195 0.332 0.621 1.158 2.261

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.