GAPDH Mouse Monoclonal Antibody [5-E10]
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
GAPDH,G3P
Swiss Prot
P04406 Human, P16858 Mouse, P04797 Rat
Cellular Locus
Cytoplasm
Host
Mouse
Species Reactivity
Human,Mouse,Rat,Zebrafish,Rabbit
Immunogen
Synthetic peptide within human GAPDH aa 10-50.
Isotype
IgM
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell, IHC-P
Positive Control
Concentration
2 mg/mL
Dilution
WB: 1:5,000-1:10,000 ;IF-Cell: 1:200-1:500 ;IHC-P:1:200-1:1,000
Purity
Protein L affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 36 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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