GAPDH Mouse Monoclonal Antibody [5-E10]
Selected Variant
EM1101
Size: 100 µL
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
GAPDH,G3P
Swiss Prot
P04406 Human, P16858 Mouse, P04797 Rat
Cellular Locus
Cytoplasm
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Zebrafish, Rabbit
Immunogen
Synthetic peptide within human GAPDH aa 10-50.
Isotype
IgM
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell, IHC-P
Positive Control
Concentration
2 mg/mL
Dilution
WB: 1:5,000-1:10,000 ;IF-Cell: 1:200-1:500 ;IHC-P:1:200-1:1,000
Purity
Protein L affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 36 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
Price
Frequently Asked Questions
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