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GAPDH Mouse Monoclonal Antibody [5-E10]

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

38 kDa BFA-dependent ADP-ribosylation substrate antibody aging associated gene 9 protein antibody Aging-associated gene 9 protein antibody BARS-38 antibody cb609 antibody EC 1.2.1.12 antibody Epididymis secretory sperm binding protein Li 162eP antibody G3P_HUMAN antibody G3PD antibody G3PDH antibody GAPD antibody GAPDH antibody Glyceraldehyde 3 phosphate dehydrogenase antibody Glyceraldehyde-3-phosphate dehydrogenase antibody HEL-S-162eP antibody KNC-NDS6 antibody MGC102544 antibody MGC102546 antibody MGC103190 antibody MGC103191 antibody MGC105239 antibody MGC127711 antibody MGC88685 antibody OCAS, p38 component antibody OCT1 coactivator in S phase, 38-KD component antibody peptidyl cysteine S nitrosylase GAPDH antibody Peptidyl-cysteine S-nitrosylase GAPDH antibody wu:fb33a10 antibody

Abbreviation

GAPDH,G3P

Swiss Prot

P04406 Human, P16858 Mouse, P04797 Rat

Cellular Locus

Cytoplasm

Host

Mouse

Species Reactivity

Human,Mouse,Rat,Zebrafish,Rabbit

Immunogen

Synthetic peptide within human GAPDH aa 10-50.

Isotype

IgM

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

MDA-MB-231 cell lysate, HeLa cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, JAR cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, HeLa, F9, zebrafish cell/tissue lysates, human liver tissue, mouse liver tissue, human spleen tissue, rat liver tissue, rat spleen tissue.

Concentration

2 mg/mL

Dilution

WB: 1:5,000-1:10,000 ;IF-Cell: 1:200-1:500 ;IHC-P:1:200-1:1,000

Purity

Protein L affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 36 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No

Curated Selection

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