ELISA Buffer Set-96T

Coating buffer: Use coating buffer to dilute the sample you will coat, add 100μL/well to high-bind plate.<br> Washing buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for washing the coated plate.<br> Blocking buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for blocking the coated plate. The samples and detection antibodies can be prepared by 1x blocking buffer which should be 541 times dilution.<br> Substrate solution: Add 100μL/well to plate.<br> Stop solution: Add 50μL/well to plate.

Store this set at 2-8°C, and protect from light. If precipitation occurs, filter to clarify before use. The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed. Below is the Shipping Statement for Kit Products.