hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-01]
Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place. Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis. Required for H3K4me1 mark at stalled replication forks. Mediates FANCD2-dependent nucleosome remodeling and RAD51 nucleofilaments stabilization at reversed forks, protecting them from nucleolytic degradation. Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Swiss Prot
O15047 Human, E9PYH6 Mouse, A0A8I6AS32 Rat
Cellular Locus
Chromosome, Nucleus
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein within C terminal human SET1A.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P
Positive Control
293T cell lysate, Jurkat cell lysate, human lung carcinoma tissue, human testis tissue, mouse hippocampus tissue.
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IHC-P: 1:400
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 186 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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