Recombinant Human Biotinidase (BTD), partial
Product Specifications
Product Name Alternative
(Biotinase)
Abbreviation
Recombinant Human BTD protein, partial
Gene Name
BTD
UniProt
P43251
Expression Region
322-397aa
Organism
Homo sapiens (Human)
Target Sequence
YHDMENPKSHLIIAQVAKNPVGLIGAENATGETDPSHSKFLKILSGDPYCEKDAQEVHCDEATKWNVNAPPTFHSE
Tag
N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Metabolism
Relevance
Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
41.3 kDa
References & Citations
"Partial biotinidase deficiency is usually due to the D444H mutation in the biotinidase gene." Swango K.L., Demirkol M., Huener G., Pronicka E., Sykut-Cegielska J., Schulze A., Mayatepek E., Wolf B. Hum. Genet. 102:571-575 (1998)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
PH33230M5 20 µg
Periostin (POSTN) (NM_001135935) Human Recombinant Protein
Sign In for Pricing
View Details