Recombinant Human Parathyroid Hormone

The protein (1 mg/ml) was lyophilized after extensive dialysis against 1.15mg sodium citrate, 7.31mg sodium chloride, 0.21 mg citric acid, 0.1117mg EDTA-Na2, 0.2mg Tween 80 and 50mg mannitol. Purity >98% as determined by RP-HPLC and SDS-PAGE.

Lyophilized product should be stored desiccated below -20°C. Upon reconstitution, product may be stored at 4°C for 2-7 days and, for future use, below -20°C. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) is added. Prepare appropriate aliquots to avoid multiple freeze-thaw cycles.

The protein (1 mg/ml) was lyophilized after extensive dialysis against 1.15mg sodium citrate, 7.31mg sodium chloride, 0.21 mg citric acid, 0.1117mg EDTA-Na2, 0.2mg Tween 80 and 50mg mannitol.

Parathyroid hormone (PTH) is a 84-amino acid polypeptide secreted by parathyroid glands that increases the concentration of calcium in the blood through its interaction with parathyroid hormone receptors in bones, kidney, and intestines. In bones PTH enhances the release of calcium from the large reservoir contained in bones, in kidney it enhances active reabsorption of calcium from distal tubules and the thick ascending limb, and in intestines it enhances the absorption of calcium by increasing the production of vitamin D and upregulating the enzyme responsible for 1-alpha hydroxylation of 25-hydroxy vitamin D, converting vitamin D to its active form (1,25-dihydroxy vitamin D) which effects the absorption of calcium (as Ca2+ ions) by the intestine via calbindin.