VAV2 (D138) polyclonal antibody

Product Specifications

Background

VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Guanine nucleotide exchange factor VAV2; VAV-2; VAV2

Swiss Prot

P52735

Reactivity

Human, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 100-150 of Human VAV2.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~108 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

VAV2 (D138) polyclonal antibody detects endogenous levels of VAV2 protein.

Applications Notes

Western blot (WB) analysis of VAV2 (D138) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (20ug) Lane2:Hela whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug)