VAV2 (D138) polyclonal antibody
Product Specifications
Background
VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Guanine nucleotide exchange factor VAV2; VAV-2; VAV2
Swiss Prot
P52735
Reactivity
Human, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 100-150 of Human VAV2.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~108 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
VAV2 (D138) polyclonal antibody detects endogenous levels of VAV2 protein.
Applications Notes
Western blot (WB) analysis of VAV2 (D138) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (20ug) Lane2:Hela whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug)
Host or Source
Rabbit
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