Mouse anti-FLAG-tag Antibody

Product Specifications

Background

Mouse monoclonal antibody to FLAG-tag

CAS Number

9007-83-4

Host

Mouse

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of FLAG-tag. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IF/IC, IP

Concentration

1mg/mL

Dilution

WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of over-expressed HA-tagged protein in 293T cell lysate.

IF/ICC description

Immunofluorescent analysis of HA-tag staining in 293T cells transfected with a HA-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .