Rat DLL1 ELISA Kit PicoKine®

DLL1 (DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 99 101 94 94 5.34 5.7% Sample 2 294 288 315 322 302 12.49 4.1% Sample 3 963 952 841 937 923 60.01 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 99 101 94 94 5.34 5.7% Sample 1 81 99 101 94 94 5.34 5.7% Sample 2 294 288 315 322 302 12.49 4.1% Sample 2 294 288 315 322 302 12.49 4.1% Sample 3 963 952 841 937 923 60.01 6.5% Sample 3 963 952 841 937 923 60.01 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 294 963 95 303 952 Standard deviation 5.18 19.11 56.82 4.56 16.06 58.07 CV (%) 6.4% 6.5% 5.9% 4.8% 5.3% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 294 963 95 303 952 Standard deviation 5.18 19.11 56.82 4.56 16.06 58.07 CV (%) 6.4% 6.5% 5.9% 4.8% 5.3% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 294 963 95 303 952 Mean (pg/ml) 81 294 963 95 303 952 Standard deviation 5.18 19.11 56.82 4.56 16.06 58.07 Standard deviation 5.18 19.11 56.82 4.56 16.06 58.07 CV (%) 6.4% 6.5% 5.9% 4.8% 5.3% 6.1% CV (%) 6.4% 6.5% 5.9% 4.8% 5.3% 6.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.118 0.207 0.334 0.558 0.975 1.516 2.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.118 0.207 0.334 0.558 0.975 1.516 2.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.118 0.207 0.334 0.558 0.975 1.516 2.05 O.D. 0.03 0.118 0.207 0.334 0.558 0.975 1.516 2.05

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.