Rabbit anti-TSC22 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to TSC22
Synonyms
KIAA1994; TGFB1I4; TSC22; TSC22 domain family protein 1; Cerebral protein 2; Regulatory protein TSC-22; TGFB-stimulated clone 22 homolog; Transforming growth factor beta-1-induced transcript 4 protein
Host
Rabbit
Reactivity
H, M, R, B, C, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TSC22. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of TSC22 expression in MCF7 (A), HeLa (B), mouse liver (C), rat liver (D) whole cell lysates.
IHC description
Immunohistochemical analysis of TSC22 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of TSC22 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
8848
Gene ID (Mouse)
21807
Gene ID (Rat)
498545
SwissProt (Human)
Q15714
SwissProt (Mouse)
P62500
SwissProt (Rat)
P62501
CAS Number
9007-83-4
Available Sizes
Curated Selection
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