Rabbit anti-TRPV5 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to TRPV5
CAS Number
9007-83-4
Synonyms
ECAC1; Transient receptor potential cation channel subfamily V member 5; TrpV5; Calcium transport protein 2; CaT2; Epithelial calcium channel 1; ECaC; ECaC1; Osm-9-like TRP channel 3; OTRPC3
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human TRPV5
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of TRPV5 expression in HepG2 (A), HT29 (B), mouse kidney (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of TRPV5 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
56302
Gene ID (Mouse)
194352
Gene ID (Rat)
116469
SwissProt (Human)
Q9NQA5
SwissProt (Mouse)
P69744
SwissProt (Rat)
Q9JIP0
Available Sizes
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