Rabbit anti-PRAS40 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PRAS40
CAS Number
9007-83-4
Synonyms
PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate
Host
Rabbit
Reactivity
H, M, R, B
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PRAS40. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of PRAS40 expression in MCF7 (A), human Testis (B) whole cell lysates.
IHC description
Immunohistochemical analysis of PRAS40 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
84335
Gene ID (Mouse)
67605
SwissProt (Human)
Q96B36
SwissProt (Mouse)
Q9D1F4
Available Sizes
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