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Rabbit anti-MKK1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to MKK1

Synonyms

MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1

Host

Rabbit

Reactivity

H, M, R, B, Mk, P, Rb

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MKK1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of MKK1 expression in HepG2 (A), NIH3T3 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of MKK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

5604

Gene ID (Mouse)

26395

Gene ID (Rat)

170851

SwissProt (Human)

Q02750

SwissProt (Mouse)

P31938

SwissProt (Rat)

Q01986

CAS Number

9007-83-4

Available Sizes

Curated Selection

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