Rabbit anti-CASK Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CASK
Synonyms
LIN2; Peripheral plasma membrane protein CASK; hCASK; Calcium/calmodulin-dependent serine protein kinase; Protein lin-2 homolog
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human CASK
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CASK expression in HEK293T (A), H460 (B), A549 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of CASK staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
8573
Gene ID (Mouse)
12361
Gene ID (Rat)
29647
SwissProt (Human)
O14936
SwissProt (Mouse)
O70589
SwissProt (Rat)
Q62915
CAS Number
9007-83-4
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
