Rabbit anti-APE1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to APE1
CAS Number
9007-83-4
Synonyms
APE; APE1; APEX; APX; HAP1; REF1; DNA- (apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1
Host
Rabbit
Reactivity
H, M, R, B, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human APE1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of APE1 expression in HeLa (A), Jurkat (B), SW480 (C), A431 (D), NIH3T3 (E), mouse kidney (F), rat lung (G) whole cell lysates.
IHC description
Immunohistochemical analysis of APE1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
328
Gene ID (Mouse)
11792
Gene ID (Rat)
79116
SwissProt (Human)
P27695
SwissProt (Mouse)
P28352
SwissProt (Rat)
P43138
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