Rabbit anti-PRDM12 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PRDM12
CAS Number
9007-83-4
Synonyms
PFM9; PR domain zinc finger protein 12; PR domain-containing protein 12
Host
Rabbit
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRDM12. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of PRDM12 expression in U87MG (A), MCF7 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PRDM12 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
59335
SwissProt (Human)
Q9H4Q4
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