Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-TGF beta 1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to TGF beta 1

CAS Number

9007-83-4

Synonyms

TGFB; Transforming growth factor beta-1; TGF-beta-1

Host

Rabbit

Reactivity

H, M, R, B, D, P, S

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TGF beta 1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of 14-3-3 zeta (pS58) expression in MCF7 (A), A549 (B), SP2/0 (C), rat liver (D), NIH3T3 (E), A431 (F) whole cell lysates.

IHC description

Immunohistochemical analysis of 14-3-3 zeta (pS58) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of 14-3-3 zeta (pS58) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

7534

Gene ID (Mouse)

22631

Gene ID (Rat)

25578

SwissProt (Human)

P63104

SwissProt (Mouse)

P63101

SwissProt (Rat)

P63102

Available Sizes

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Magic™ Membrane Protein Human TMEM249 (Transmembrane protein 249) Expressed in vitro E.coli expression system, Full Length
MPX1440K Each

Magic™ Membrane Protein Human TMEM249 (Transmembrane protein 249) Expressed in vitro E.coli expression system, Full Length

Sign In for Pricing
View Details
Gabrb2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
21159116 3x 1.0 µg

Gabrb2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

Sign In for Pricing
View Details
ERBB2 Antibody
AM8446b 400 µL

ERBB2 Antibody

Sign In for Pricing
View Details
Recombinant Myosin Light Chain 3 Monoclonal Antibody
AN301607L-01 50 µL

Recombinant Myosin Light Chain 3 Monoclonal Antibody

Sign In for Pricing
View Details
Recombinant Myosin Light Chain 3 Monoclonal Antibody
AN301607L-02 100 µL

Recombinant Myosin Light Chain 3 Monoclonal Antibody

Sign In for Pricing
View Details
ASCL2 Protein Vector (Rat) (pPB-C-His)
PV254906 500 ng

ASCL2 Protein Vector (Rat) (pPB-C-His)

Sign In for Pricing
View Details