Rabbit anti-SMAD2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SMAD2
CAS Number
9007-83-4
Synonyms
MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
Host
Rabbit
Reactivity
H, M, R, B, C, Mk, P, Rb, S, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SMAD2. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of SMAD2 expression in SHSY5Y (A), K562 (B), COLO205 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of SMAD2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of SMAD2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4087
Gene ID (Mouse)
17126
Gene ID (Rat)
29357
SwissProt (Human)
Q15796
SwissProt (Mouse)
Q62432
SwissProt (Rat)
O70436
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS1374967-01 0.05 mg (E-Coli)
Recombinant Gibberella zeae Protein CSN12 homolog (CSN12), partial
Ask
View Details 
MBS1374967-02 0.05 mg (Yeast)
Recombinant Gibberella zeae Protein CSN12 homolog (CSN12), partial
Ask
View Details MBS1374967-03 0.05 mg (Baculovirus)
Recombinant Gibberella zeae Protein CSN12 homolog (CSN12), partial
Ask
View Details 
MBS1374967-04 0.2 mg (E-Coli)
Recombinant Gibberella zeae Protein CSN12 homolog (CSN12), partial
Ask
View Details MBS1374967-05 0.5 mg (E-Coli)
Recombinant Gibberella zeae Protein CSN12 homolog (CSN12), partial
Ask
View Details MBS1374967-06 0.2 mg (Yeast)
Recombinant Gibberella zeae Protein CSN12 homolog (CSN12), partial
Ask
View Details