Rabbit anti-PPP2R5A Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PPP2R5A
CAS Number
9007-83-4
Synonyms
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform; PP2A B subunit isoform B'-alpha; PP2A B subunit isoform B56-alpha; PP2A B subunit isoform PR61-alpha; PR61alpha; PP2A B subunit isoform R5-alpha
Host
Rabbit
Reactivity
H, M, R, B, Mk, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PPP2R5A. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PPP2R5A expression in HeLa (A), NIH3T3 (B), H9C2 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PPP2R5A staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5525
Gene ID (Mouse)
226849
SwissProt (Human)
Q15172
SwissProt (Mouse)
Q6PD03
Available Sizes
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