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Rabbit anti-PPP2R5A Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PPP2R5A

CAS Number

9007-83-4

Synonyms

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform; PP2A B subunit isoform B'-alpha; PP2A B subunit isoform B56-alpha; PP2A B subunit isoform PR61-alpha; PR61alpha; PP2A B subunit isoform R5-alpha

Host

Rabbit

Reactivity

H, M, R, B, Mk, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PPP2R5A. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PPP2R5A expression in HeLa (A), NIH3T3 (B), H9C2 (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PPP2R5A staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5525

Gene ID (Mouse)

226849

SwissProt (Human)

Q15172

SwissProt (Mouse)

Q6PD03

Available Sizes

Frequently Asked Questions

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