Rabbit anti-Speedy A Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Speedy A
CAS Number
9007-83-4
Synonyms
SPDY1; SPY1; Speedy protein A; Rapid inducer of G2/M progression in oocytes A; RINGO A; hSpy/Ringo A; Speedy-1; Spy1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Speedy A
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Speedy A expression in PC3 (A), U87 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Speedy A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
245711
Gene ID (Mouse)
70891
Gene ID (Rat)
192209
SwissProt (Human)
Q5MJ70
SwissProt (Mouse)
Q5IBH7
SwissProt (Rat)
Q8R496
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