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Rabbit anti-Borealin Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Borealin

CAS Number

9007-83-4

Synonyms

PESCRG3; Borealin; Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Borealin

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Borealin expression in HEK293T (A) whole cell lysates.

IHC description

Immunohistochemical analysis of Borealin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

55143

Gene ID (Mouse)

52276

Gene ID (Rat)

500545

SwissProt (Human)

Q53HL2

SwissProt (Mouse)

Q8BHX3

SwissProt (Rat)

Q6AXW0

Available Sizes

Frequently Asked Questions

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