Mouse anti-Smooth Muscle Actin Antibody
Product Specifications
Background
Mouse monoclonal antibody to Smooth Muscle Actin
CAS Number
9007-83-4
Synonyms
ACTSA; ACTVS; Actin aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Smooth Muscle Actin. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1mg/mL
Dilution
WB (1/10000 - 1/100000), IH (1/1000 - 1/2000)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Smooth Muscle Actin expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Smooth Muscle Actin staining in mouse cecal formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
59
SwissProt (Human)
P62736
Available Sizes
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