Rabbit anti-Herc3 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Herc3
CAS Number
9007-83-4
Synonyms
KIAA0032; Probable E3 ubiquitin-protein ligase HERC3; HECT domain and RCC1-like domain-containing protein 3
Host
Rabbit
Reactivity
H, M
Immunogen
Recombinant full length protein of human Herc3
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Herc3 expression in A431 (A), mouse lung (B) whole cell lysates.
IHC description
Immunohistochemical analysis of Herc3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
8916
SwissProt (Human)
Q15034
Available Sizes
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