CCL5/RANTES Antibody

Product Specifications

Product Name Alternative

CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228

Reactivity

Human

Immunogen

RANTES

Target

CCL5 / RANTES

Clonality

Monoclonal

Clone

8.PP.11

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein A purified

Concentration

1 mg/ml

Dilution

ELISA (2 - 4 μg/ml), IHC, IHC-P (5 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Neutralization: 5-7 ug/ml to yield maximum inhibition (ND50) of hRANTES at 100 ng/ml) .