Rabbit anti-ERK7 (pT175/Y177) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ERK7 (pT175/Y177)

CAS Number

9007-83-4

Synonyms

ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8

Host

Rabbit

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ERK7. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ERK7 (pT175/Y177) expression in Jurkat (A) whole cell lysates.

IHC description

Immunohistochemical analysis of ERK7 (pT175/Y177) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.