PD-L1 Antibody / B7-H1 / CD274

This antibody reacts to human PD-L1 (CD274, B7-H1) .

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:200-1:1000, Western blot: 1:500-1:1000, Immunocytochemistry: 1:200-1:500

UniProt

Q9EP73

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the C-terminus of human PD-L1 was used as the immunogen for the recombinant PD-L1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM320

Type

Recombinant

Applications

WB, IHC-P, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant PD-L1 antibody at -20oC.

Limitations

This recombinant PD-L1 antibody is available for research use only.

Storage Conditions

Store the recombinant PD-L1 antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant PD-L1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic

Image Legend

Western blot testing of human tumor cell line lysate with recombinant PD-L1 antibody at 1:1000. DU145 and LNCaP are prostate cancer cell lines with very minor PD-L1 expression. Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated) .