HO-2 Antibody / Heme oxygenase 2 / HMOX2

Heme oxygenase 2 (HMOX2), also known as HO-2, is an enzyme that in humans is encoded by the HMOX2 gene. It is mapped to 16p13.3. HMOX2 belongs to the heme oxygenase family. Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P30519

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids H45-A301) was used as the immunogen for the HO-2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the HO-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HO-2 antibody is available for research use only.

Storage Conditions

After reconstitution, the HO-2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HO-2 antibody should be determined by the researcher.

Location

Cytoplasmic (ER)

Image Legend

IHC staining of FFPE human appendiceal adenocarcinoma tissue with HO-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.