Anti-Mouse CD317 (927) In Vivo Antibody - Low Endotoxin
Product Specifications
CAS Number
9007-83-4
Specifications
Clone 927 activity is directed against murine CD317 (BST2; PDCA-1).
Host
Rat
Species Reactivity
Mouse
Target
CD317
Isotype
IgG2a kappa
Clone
927
Applications
Depletion, Functional Assays, Flow Cytometry, Immunocytochemistry, Immunofluorescence Microscopy
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
≥ 2.0 mg/mL
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
Sterile, preservative-free, solution in PBS. BSA and Azide free.
Antigen Distribution
Murine CD317 is expressed by IFN-producing cells in naïve mouse spleen and by a wide variety of cell lines including T cells, mast cells, B cells, fibroblast cells, and pluripotent embryonal carcinoma cells. Additionally, mice challenged by influenza or other stimuli (CpG, LPS, murine CMV, poly(I:C), and imiquimod) express CD317 in DC and other myeloid cells as well as T cells, B cells, NKT cells, and some NK cells. CD317 is also expressed on CD138+ plasma cells in naïve mice and is upregulated by viral stimulation.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244
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