Anti-SUR2A Antibody

Mouse Monoclonal Antibody specific to SUR2A

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Sulfonylurea receptor 2

Gene Name

Abcc9

NCBI Gene ID

UniProt

P70170

Accession Number

NP_001038185.1

Cellular Locus

Membrane, Multi-pass membrane protein

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Fusion protein corresponding to aa 1505-1546 (SSIVDAGLVLVFSEGILVECDTGPNLLQ HKNGLFSTLVMTNK, cytoplasmic C- terminus) of mouse SUR2A (accession no. P70170).

Target Antigen

ATP-binding cassette sub-family C member 9

Target

SUR2A

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with KCNJ11. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~120kDa is detected. <br><br>Positive control: Mouse brain lysate. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes mouse and rat SUR2A. It does not cross-react with SUR2B.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Sulfonylurea receptors (SUR) are membrane proteins that are molecular targets of the sulfonylurea class of antidiabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the SUR is to sense intracellular levels of ATP and ADP and facilitate the opening or closing of its associated Kir6.x potassium channel, thus monitoring the energy balance within cells.