Placental Alkaline Phosphatase Antibody / ALPP / PLAP

Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P05187

Host

Mouse

Reactivity

Human

Immunogen

C-terminal amino acids KARDRKAYTVLLYGNGPGYVLKDGARPDVTE were used as the immunogen for the Placental Alkaline Phosphatase antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

6C7G3

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Placental Alkaline Phosphatase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Placental Alkaline Phosphatase antibody is available for research use only.

Storage Conditions

After reconstitution, the Placental Alkaline Phosphatase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Placental Alkaline Phosphatase antibody should be determined by the researcher.

Location

Cytoplasmic and cell surface

Image Legend

Immunofluorescent staining of FFPE human placental tissue with Placental Alkaline Phosphatase antibody (green) and DAPI nuclear stain (blue) . HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.