Human Insulin Recombinant
Recombinant human Insulin is a disulfide-linked homodimeric protein consisting of 51 amino acid residues, and migrates as an approximately 6 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Insulin mature chain was expressed inE. coli.
Product Specifications
UniProt
P01308
Endotoxin
Endotoxin level was found to be < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Purity
≥ 95% by SDS-PAGE and HPLC
Format
Lyophilized protein
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.
Shipping Conditions
Gel Packs or Dry Ice
Storage Conditions
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Calculated Molecular Weight
6 kDa
Formulation
Lyophilized from a 0.2 µm filtered aqueous solution with no additives
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