Recombinant Mouse IL3
IL3 is produced mainly by T cells following cell activation by antigens and mitogens, but also by keratinocytes, natural killer cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial-derived recombinant IL3 shows that glycosylation is not required for the activity of IL3. IL-3 sequences are evolutionarily less well conserved. Human and murine IL3 show approximately 29% homology at the protein level while murine and rat IL3 show approximately 54% homology. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells, and various myeloid leukemia cells. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein. Recombinant mouse IL3 is a monomer of 15 kDa.
Product Specifications
CAS Number
9000-83-3
Gene Name
IL3
Gene Aliases
Gene ID
16187
Accession Number
P01586
Organism
Mouse
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method
Purity
>95% as determined by SDS-PAGE
Solubility
Function
The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells was found to be in the <0.05 ng/mL
Molecular Weight
15
Shipping Conditions
Ambient Temperature
Storage Conditions
Appearance
Lyophilized Powder
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS
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