GLS1 Inhibitor Screening Assay Kit

Kidney-type glutaminase (GLS1) is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of L-glutamine to L-glutamate and ammonia. GLS1 is primarily expressed in the brain and kidney where it catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. It plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney.

Catalog NumberComponentAmountStorage71102GLS1, His-tag**1 µg-80 °CAvoidfreeze/thawcycles!L-Glutamine (100 mM) 100 µl-20 °CNAD+ (20mM) 1 ml-20 °CCoupling reagent10 µl-20 °C799484X GLS assay buffer*2.5 ml-20 °CCB-839 (100 µM) 20 µl-20 °C7968596-well black microplate1Room Temp.** The concentration of GLS1 is lot-specific and will be indicated on the tube containing the enzyme.* Add 20 µl of 0.5 M DTT to 2.5 ml 4X GLS1 assay buffer before use.

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.