UBR2 Antibody / E3 ubiquitin-protein ligase UBR2

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. (UniProt)

Product Specifications

CAS Number

9007-83-4

Specifications

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q8IWV8

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human UBR2 protein was used as the immunogen for the UBR2 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-UBR2-1D12

Applications

IP, FACS, IF, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the UBR2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This UBR2 antibody is available for research use only.

Storage Conditions

Aliquot the UBR2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the UBR2 antibody should be determined by the researcher.

Location

Nucleus, cytoplasm

Image Legend

IHC staining of FFPE human thyroid tissue with UBR2 antibody (clone PCRP-UBR2-1D12) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.