Recombinant Prostate Specific Antigen Antibody / PSA

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA) . This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT) . A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This mAb makes an excellent pair with clone 1A7G6B6 for PSA tests.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P07288

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human KLK3/PSA protein was used as the immunogen for the recombinant Prostate Specific Antigen antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2a κ

Clone

RKLK3/4753

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Prostate Specific Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Prostate Specific Antigen antibody is available for research use only.

Storage Conditions

Store the recombinant Prostate Specific Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Prostate Specific Antigen antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human prostate carcinoma with recombinant Prostate Specific Antigen antibody (clone rKLK3/4753) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.