ACE Antibody / Angiotensin I Converting Enzyme

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, ELISA: order Ab without BSA for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P12821

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 652-788 from the human protein was used as the immunogen for the ACE antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

ACE/3762

Applications

WB, ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the ACE antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ACE antibody is available for research use only.

Storage Conditions

Store the ACE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the ACE antibody should be determined by the researcher.

Location

Cell surface, cytoplasm

Image Legend

IHC staining of FFPE human colon with ACE antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.