FLI1 Antibody

Recognizes a protein of 51kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewings Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order Ab without BSA for coating

UniProt

Q01543

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 107-261 from the human protein was used as the immunogen for the FLI1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

FLI1/3183

Applications

ELISA

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the FLI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This FLI1 antibody is available for research use only.

Storage Conditions

Store the FLI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the FLI1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using FLI1 antibody (clone FLI1/3183) . These results demonstrate the foremost specificity of the FLI1/3183 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.