Basic Cytokeratin Antibody (HMW / Type II)

This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, , Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q01546

Host

Mouse

Reactivity

Human

Immunogen

Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction was used as the immunogen for this Basic Cytokeratin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

DE-SQ

Applications

IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Basic Cytokeratin antibody is available for research use only.

Storage Conditions

Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Cytokeratin Basic antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human tonsil with Basic Cytokeratin antibody (clone DE-SQ) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.