PPP3CA Antibody / Calcineurin
Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL
UniProt
Q08209
Host
Mouse
Reactivity
Human
Immunogen
A recombinant full-length Calcineurin A protein was used as the immunogen for the PPP3CA antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
BF1.1
Applications
WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the PPP3CA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PPP3CA antibody is available for research use only.
Storage Conditions
Store the PPP3CA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/ppp3ca-antibody-calcineurin-bf11-v8010
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the PPP3CA antibody should be determined by the researcher.
Image Legend
SDS-PAGE analysis of purified, BSA-free PPP3CA antibody (clone BF1.1) as confirmation of integrity and purity.
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