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ARF1 Antibody / ADP-ribosylation factor 1

The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P84077

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids SNQLRNQ were used as the immunogen for this ARF1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

3F1

Applications

IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the ARF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ARF1 antibody is available for research use only.

Storage Conditions

Store the ARF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the ARF1 antibody should be determined by the researcher.

Location

Cytoplasmic (Golgi)

Image Legend

SDS-PAGE analysis of purified, BSA-free ARF1 antibody (clone 3F1) as confirmation of integrity and purity.

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