PD-L1 Antibody / B7-H1 / CD274
PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA: order BSA-free format for coating, Flow cytometry: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL
UniProt
Q9NZQ7
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 39-191 from the human protein was used as the immunogen for this PD-L1 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
PDL1/2743
Applications
ELISA, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the PD-L1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PD-L1 antibody is available for research use only.
Storage Conditions
Store the PD-L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the PD-L1 antibody should be determined by the researcher.
Location
Cell surface, cytoplasm
Image Legend
Flow cytometry testing of human Jurkat cells with PD-L1 antibody (clone PDL1/2743) ; Red=isotype control, Blue= cells alone, Orange= PD-L1 antibody.
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