GATA3 Antibody

GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas.'Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2 µg/mL

UniProt

P23771

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 357-436) was used as the immunogen for the GATA3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

GATA3/2445

Applications

ELISA, FACS, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the GATA3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This GATA3 antibody is available for research use only.

Storage Conditions

Store the GATA3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the GATA3 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using GATA3 antibody (clone GATA3/2445) . These results demonstrate the foremost specificity of the GATA3/2445 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.