AKT1 Antibody
Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P31749
Host
Mouse
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 1-200 from the human protein were used as the immunogen for the AKT1 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
AKT1/2784
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This AKT1 antibody is available for research use only.
Storage Conditions
Store the AKT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the AKT1 antibody should be determined by the researcher.
Location
Nucleus, plasma membrane, cytoplasm
Image Legend
Western blot testing of mouse NIH3T3 cell lysate with AKT1 antibody. Predicted molecular weight ~56 kDa.
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