What is the catalog number for PHB Antibody / Prohibitin?

The catalog number for PHB Antibody / Prohibitin is V7616IHC-7ML.

What sizes are available for PHB Antibody / Prohibitin?

PHB Antibody / Prohibitin is available in the following size(s): 7 mL.

Is PHB Antibody / Prohibitin in stock?

PHB Antibody / Prohibitin is currently made to order / not held in immediate stock. Contact us for lead time.

PHB Antibody / Prohibitin

This antibody recognizes a protein of 30kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P35232

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

PHB/3228

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the PHB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PHB antibody is available for research use only.

Storage Conditions

Store the PHB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Applications Notes

Optimal dilution of the PHB antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human liver tissue with PHB antibody (clone PHB/3228) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.