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14-3-3 epsilon Antibody / YWHAE

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. The YWHAE gene which encodes 14-3-3 epsilon has also been identified as a possible susceptibility gene for schizophrenia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P62258

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the 14-3-3 epsilon antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CPTC-YWHAE-1

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the 14-3-3 epsilon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This 14-3-3 epsilon antibody is available for research use only.

Storage Conditions

Store the 14-3-3 epsilon antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear, cytoplasmic

Image Legend

Western blot testing of human 1) HEK293, 2) PC-3 and 3) A431 lysate with 14-3-3 epsilon antibody. Predicted molecular weight ~28 kDa.

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