14-3-3 epsilon Antibody / YWHAE
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. The YWHAE gene which encodes 14-3-3 epsilon has also been identified as a possible susceptibility gene for schizophrenia.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P62258
Host
Mouse
Reactivity
Human
Immunogen
Full length recombinant human protein was used as the immunogen for the 14-3-3 epsilon antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
CPTC-YWHAE-1
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the 14-3-3 epsilon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This 14-3-3 epsilon antibody is available for research use only.
Storage Conditions
Store the 14-3-3 epsilon antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear, cytoplasmic
Image Legend
Western blot testing of human 1) HEK293, 2) PC-3 and 3) A431 lysate with 14-3-3 epsilon antibody. Predicted molecular weight ~28 kDa.
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