SUMO2/3 Antibody

The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, 2 and 3, belong to the ubiquitin-like protein family. SUMO proteins are synthesized as precursors that undergo processing before conjugation to target proteins. Sumoylation targets for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. SUMO2/3 contribute to a greater percentage of protein modification than does SUMO1 and they can form polymeric chains. In addition, SUMO3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimerï¿½s disease.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human SUMO2 protein was used as the immunogen for this SUMO2/3 antibody. This antibody reacts with both SUMO2 and SUMO3.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

S23MT-1

Applications

IHC-P

Purity

Protein G purified

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the SUMO2/3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This SUMO2/3 antibody is available for research use only.

Storage Conditions

Store the SUMO2/3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Applications Notes

Titering of the SUMO2/3 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Predominantly nuclear with some cytoplasmic staining

Image Legend

IHC analysis of human tonsil with SUMO2/3 antibody (clone S23MT-1) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.