PD-L1 Antibody

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL at RT

UniProt

Q9NZQ7

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 39-191 from the human protein was used as the immunogen for the PD-L1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PDL1/2746

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Biotin Conjugate

Buffer

0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the PD-L1 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Limitations

This PD-L1 antibody is available for research use only.

Storage Conditions

Store the PD-L1 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

Formulation

0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the PD-L1 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE lung carcinoma with biotin-conjugated PD-L1 antibody (clone PDL1/2746) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.